Effect of i.n. administration of gliadin on frequency of γδ T cells.

<p>(A) Proportion of γδ T cells within T (CD3-gated) cells in mucosal (NALT, MLN, PLN) and non-mucosal compartments (Spleen, ILN) after i.n. gliadin (white bars) or OVA (black bars) administration in 8-week-old NOD female mice. Panels C and D show further analysis of the γδ T cells according to their CD8 expression; lymphoid organs and bars as ad A. (C) Proportion of CD8^- γδ T cells expressed as percentage of CD3⁺ T cells. (D) Proportion of CD8⁺ γδ T cells expressed as percentage of CD3⁺ T cells. (B) Example FACS analysis of γδ T cells (CD3-gated) in MLN and PLN of NOD mice treated i.n. with gliadin or OVA. Individual measurements were performed on cells pooled from 2–3 experimental animals. Data are expressed as mean values ±SEM and represent an example of two independent experiments. *p<0.05, **p<0.01, ***p<0.001.