GLuc expression in replicon-defective transfected cells infected with varying PFUs of alphaviruses.

<p>BHK-21 cells were transfected with pVaXJ-GLucΔnsp4 using Lipofectamine 2000 reagent, 6 h before mock-infection or infection with varying PFU levels of alphaviruses. GLuc activity was measured 44 h post-infection. Each data point represents the mean ±SEM of three independent experiments. RLU, relative light units.