Effect of host factors knockdown on Gluc expression in A549-5Ps cells.

<p>A549-5Ps cells were transfected with 10nM negative control siRNA (NC), Tat-SF1 siRNA and cyclinT1 siRNA, respectively. (A) Gluc activity in supernatants was quantified. (B and C) Total cellular RNAs were extracted to quantify mRNA level of Tat-SF1 and cyclinT1 by real-time RT-PCR. Error bars standard error of the mean of four independent experiments. (D) A549-5Ps cells were transducted with shRNAs targeting candidate genes as indicated. The Gluc activity (top panel) and cell viability (middle panel) were quantified, and GLuc activity normalized with cell viability was presented in the bottom panel.