EGFP and GLuc expression in replicon-transfected cells.

<p>BHK-21 cells were transfected with pVaXJ-EGFP, pVaXJ-EGFPΔnsp4, pVaXJ-GLuc, or pVaXJ-GLucΔnsp4 using Lipofectamine 2000 reagent. (A), (B) EGFP expression was examined 48 h post-transfection using an Olympus IX51 fluorescence microscope. Green color indicates EGFP, and blue color indicates nucleus. (C) GLuc activity was measured at different time points (from 18–170 h) post-transfection using the BioLux™ Gaussia Luciferase Assay Kit and a luminometer. Each data point represents the mean ±SEM of three independent experiments. RLU, relative light units.