Secreted GLuc activity in cultured transfected L3.

<p>L3 = cultured in absence of cells. Cells = BESM cells alone. L3 + cells = L3 cultured and transfected over BESM monolayer. Columns represent mean activity and error bars standard deviation of data obtained from three biological replicates (i.e. independent transfections carried out individual transwells) per treatment. The transfections employing L3 alone and L3 + cells contained approximately 100 larvae per transwell. Values represent net counts calculated by subtracting the relative light units (RLU) seen in media collected from untransfected cells, L3 and cells + L3 respectively.