Knockdown of cyclin K in developing embryos.

<p>(<b>A</b>) Semiquantitative RT-PCR analysis of cyclin K mRNA levels in control (GFPi) and test (cycKi) groups. (<b>B</b>) Western Blot analysis of cyclin K protein levels in control (GFPi) and test (cycKi) groups. (<b>C</b>) Morphology of adult <i>Artemia</i>: cyclin K RNAi (a) and GFP RNAi (b); Offspring produced by cyclin K RNAi (c) and GFP RNAi (d) <i>Artemia</i>; Trypan Blue staining of embryos entering utrus for four days reproduced by cyclin K RNAi (e) and GFP RNAi (f) <i>Artemia</i>. The bars in (a) and (b) represent 30 mm. The bars in (c) represent 215 µm. The bars in (d–f) represent 150 µm. (<b>D</b>) DAPI staining of early development embryos. The samples are eggs having entered the uterus for 12, 24, 32 and 48 h respectively. The upper two panels are GFP RNAi groups (GFPi); the lower two panels are cyclin K RNAi groups (RNAi). (<b>E</b>) TUNEL assay of embryos at the same stages of development as shown in D, 8-µm frozen sections were prepared and DNA strand breaks were detected by the TUNEL assay. Arrows indicated positive signals in cyclin K RNAi embryos. The bars in both (D) and (E) indicate 35 µm.