Semiquantitative RT-PCR analysis of cyclin K expression in <i>Artemia</i>.

<p>(<b>A</b>) Cyclin K expression during <i>Artemia</i> development: postdiapause development stages (0, 4 and 8 h) represent postdiapause cysts incubated for 0, 4 and 8 h, respectively. Larval and post-larval development stages (0.2, 0.5 and 1.0 cm) represent larva of body length 0.2, 0.5 and 1.0 cm, respectively. ANE: adults without eggs. (<b>B</b>) Cyclin K expression in cephalothorax (ce) and ovisacs (ov) of both oviparous and ovoviviparous animals. 0 h is as the same sample as 0 h in (A) and represents postdiapause cysts incubated for 0 h. (<b>C</b>) Expression of cyclin K and Rpb1 in oocytes and embryos of both oviparous and ovoviviparous developmental pathways. EO, early oocytes; LO, late oocytes; 1dE, 3dE and 4dE represent embryos having entered the uterus for 1 day, 3 days and 4 days, respectively. (<b>D</b>) Expression of cyclin K and Rpb1 during the hatching process of diapause embryos (which includes the diapause embryo, postdiapause embryo, 0- to 14-h incubated embryos and nauplius); α-tubulin was used as a loading control.