The relative amounts of <i>ArSLK</i> mRNA detected by real-time PCR.

<p>The mRNA amounts were normalized to those of <i>tubulin</i> mRNA. (<b>A</b>) The relative amounts of <i>ArSLK</i> mRNA in oocytes and embryos of ovoviviparous and oviparous <i>Artemia</i>. EO, early oocytes; LO, late oocytes; EE, early embryos; LE, late embryos; Naup, nauplius larvae; Dp, diapause cysts. (<b>B</b>) The relative amounts of <i>ArSLK</i> mRNA during the hatching process of <i>Artemia</i>. Dp, diapause cysts; Pd, post-diapause cysts. The times (h) represent the incubation times of the post-diapause embryos. (<b>A, B</b>) Data are represented as mean <u>+</u> SE of n = 3 independent repeats. Statistical analyses of the differences between expression levels in the two pathways were performed by two-tailed, paired Student's <i>t</i> tests. No significant differences (<i>P</i><0.05) were detected.