The effect of MOG to stimulate basal insulin secretion was independent of changes in cytosolic Ca²⁺ and oxygen consumption.

<p>A. 200 µM MOG did not increase cytosolic Ca²⁺ in dissociated rat islet cells. Arrows indicate addition of 11 mM glucose (black trace) and 200 µM MOG (gray trace). B. 200 µM MOG (white squares) did not affect basal O₂ consumption in INS-1 cells. Incubation with and without MOG started 30 min prior to O₂ consumption measurements. Arrows indicate addition of 5 µM oligomycin (O) to inhibit respiration and 100 µM dinitrophenol (DNP) to stimulate maximal respiration. C. 200 µM MOG did not affect glucose-stimulated O₂ consumption in INS-1 cells. Arrow indicates addition of 2 mM glucose (black diamonds) and 12 mM glucose alone (white squares) or with 200 µM MOG (white triangles). A. Average signal from more than 10 cells per condition. Representative of 3 separate measurements. B and C. n = 4 from single experiment repeated three times.