Scavenging ROS with NAC (A, B) or Resveratrol (C) blocked MOG, ß-OHB and glucose-stimulated insulin secretion. The effect of MOG to stimulate basal (2 mM glucose) insulin secretion was not enhanced by the prescence of ß-OHB (D).

<p>A. 3 mM NAC (black bars) inhibited 10 mM ß-OHB-, 100 µM MOG- and 8 mM glucose-stimulated insulin secretion from INS-1 cells. B. 10 mM ß-OHB-stimulated insulin secretion was inhibited by NAC in a concentration-dependent manner (0.3–3 mM). C. 100 µM resveratrol (black bars) inhibited 10 mM ß-OHB-, 100 µM MOG- and 8 mM glucose-stimulated insulin secretion from INS-1 cells. D. The increase in insulin secretion stimulated by addition of ß-OHB and MOG together was equal to the sum of their individual effects. A–D. n = 9–12 per condition from 3 separate experiments. * p<0.005 compared to 2G control. A, C. # p<0.005 compared to its own control. B. + p,0.005 compared to no NAC. D. # p<0.005 compared to ß-OHB or MOG added separately.