The DG kinase inhibitor R59949, failed to mimic the effects of MOG to stimulate insulin secretion from INS-1 ß-cells at 2 mM glucose.

<p>0.2 mM MOG (hatched bar) increased basal (2 mM glucose) insulin secretion from INS-1 cells 2.5-fold while 12 mM glucose resulted in a 3.5-fold increase (first black bar). The effect of the DG kinase inhibitor R59949 to stimulate basal insulin secretion (white bars) was small and inconsistent over the concentration range tested (0.25–10 µM) compared to the MOG-stimulated increase in basal release (hatched bar). R59949 increased glucose-stimulated insulin release 2-fold at low concentration (0.25 µM) with higher concentrations (1–10 µM) having no stimulatory effect compared to the control (black bars). N = 9 from 3 separate experiments. *p<0.05; **p<0.005 compared to control basal value. #p<0.005 compared to high glucose control.