Quantitative analysis of KSHV BAC36 wt, RTA_1st and BAC RTA_all recombinant viruses infected PBMCs at 1dpi, 2dpi, 4dpi and 7dpi.

<p>(A) Immunofluorescence assay for PBMCs infected by KSHV BAC36 wt, RTA_1st and RTA_all recombinant viruses at 2 dpi, 4 dpi and 7 dpi. Uninfected and infected PBMCs at 2 dpi, 4 dpi and 7 dpi were stained for LANA protein expression. PBMCs expressed GFP, indicating the presence of viral genome. (B, C). Quantitative analysis for determination of latent and lytic infection in PMBC cells infected by KSHV BAC36 wt, RTA_1st and RTA_all recombinant viruses. Total RNAs were extracted, treated with DNase I, and reverse transcribed to cDNA after 1 dpi, 2 dpi, 4 dpi and 7dpi. Quantitative Real-time PCR analysis with the primers for LANA and RTA was performed using StepOnePlus Real-Time PCR System. Error bars indicate standard deviations from three separate experiments.