Transfection of 293 cells with BAC36 wt, BAC RTA_1st and BAC RTA_all DNAs.

<p>(A) Cells were transfected with BAC36 wt, BAC RTA_1st and BAC RTA_all DNAs. GFP expression levels were monitored by fluorescent microscopy 2 days after transfection, and the transfected cells were split and selected with Hygromycin B. The hygromycin-resistant cells were pooled and passed three to four times. The homogenous population of GFP-positive cells harboring KSHV wild-type and mutant genomes were obtained. (B). Immunofluorescence analysis for LANA in BAC36-293 BAC RTA_1st-293 and BAC RTA_all-293 cells.