Kinetics of MHC class II expression in PBMCs with KSHV primary infection.

<p>Quantitative real-time PCR analysis of (<b>A</b>) MHC II and LANA transcripts, and (<b>B</b>) CIITA pIII and pIV promoter transcripts, in PBMC with GFP-KSHV infection. The levels of HLA-DQαβ were enlarged on the right panel A. Total RNA was isolated from cells with GFP-KSHV infection for 0, 1, 2, 4 and 7 days. Real-time PCR was performed as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003751#s4" target="_blank">Materials and Methods</a>. The relative levels of MHCII, LANA and CIITA transcripts were calculated by the cycle threshold (ΔΔCt) values and shown by the fold change compared to mock (day 0) after normalized with GAPDH internal control. All samples were tested in triplicate and the calculation of the mean and standard deviation from two separate experiments. The mock cells without or with GFP-KSHV infection indicated by the relative intensity of LANA were quantified by qPCR shown in the figure.