Quantitative-PCR analysis of viral DNA replication in Sf9 cells transfected by recombinant AcMNPV bacmids with e25 deleted or under control of ie1 promoter.

Analysis of the replication capacity of the SIVFIV CA-p1-NC(1–9) chimera in permissive 293T cells by detecting the nuclear circular forms of unintegrated viral DNA.

María J. Esteva (668438)
José L. Affranchino (668439)
Silvia A. González (668440)

December 2014

(A) Left panel: Detection of late RT products at 48 h post-infection of 293T cells with VSV-G-pse...

Viral transduction efficiency determined via FACS and relative gene expression determined via quantitative real-time PCR.

Dong-Sik Ham (485770)
Juyoung Shin (485771)
Ji-Won Kim (174475)
Heon-Seok Park (485772)
Jae-Hyoung Cho (485773)
Kun-Ho Yoon (174472)

November 2013

(A) Various viral titers were tested using green fluorescent protein (GFP) as a reporter gene. Sa...

Knockdown of Cdc6 increases viral DNA replication.

Tamara Evans Braun (166141)
Emma Poole (166148)
John Sinclair (166153)

May 2012

HFFFs were transfected with siRNA Cdc6 or a scramble control in the presence of serum. 48 hours p...

Analysis of viral replication in Sf9 cells transfected/infected with AcMNPV recombinants with e25 under control of alternative promoters.

Xiao-Chun Luo (422940)
Shan-Shan Wang (233149)
Jie Zhang (64655)
Duo-Duo Qian (422941)
Si-Min Wang (422942)
Lu-Lin Li (422943)

June 2013

(A) Fluorescence microscopy of Sf9 cells transfected with vAcPH-gfp, vAce25ko-PH-...

Analysis of late gene expression in Sf9 cells transfected by recombinant AcMNPV bacmids with e25 deleted or under control of alternative promoters.

Xiao-Chun Luo (422940)
Shan-Shan Wang (233149)
Jie Zhang (64655)
Duo-Duo Qian (422941)
Si-Min Wang (422942)
Lu-Lin Li (422943)

June 2013

(A) Schematic maps of the structures of the recombinant AcMNPV bacmids with e25 mutants. A...

Characterization of recombinant bacmids and viruses.

Shufen Li (746508)
Manli Wang (646363)
Shu Shen (746509)
Zhihong Hu (316172)
Hualin Wang (514306)
Fei Deng (170132)

May 2015

(A) Construction of fp25k knockout bacmid. The fp25k gene in AcBacΔcc bacmid...

Virus growth and viral DNA replication analysis curves.

Huan Yu (140826)
Jiao Meng (482119)
Jian Xu (31545)
Tong-xian Liu (5664841)
Dun Wang (525747)

August 2015

A: Virus growth curves as determined by TCID50 endpoint dilution assays. HzAM1 cells w...

Viral DNA replication and gene transcription in cells transfected with vA4prm-VP39-EGFP bacmid DNA.

Jun Zhang (48506)
Xue-Mei Chen (130273)
Chun-Dong Zhang (629729)
Qian He (248656)
Zhan-Qi Dong (629730)
Ming-Ya Cao (629731)
Xiao-Long Dong (515229)
Cai-Xia Pan (515231)
Cheng Lu (57646)
Min-Hui Pan (515234)

September 2014

(A) Total cellular DNA was extracted from BmN-SWU1 and BmN-SWU2 cells at indicated times after vA...

Virus titer determination and qPCR analysis of BV genomic DNA copy number in the supernatant.

Shufen Li (746508)
Manli Wang (646363)
Shu Shen (746509)
Zhihong Hu (316172)
Hualin Wang (514306)
Fei Deng (170132)

May 2015

Sf9 cells were infected with vAcΔcc or vAcΔccΔfp25k at an MOI of 5. The supe...

Quantitative analysis of KSHV BAC36 wt, RTA1st and BAC RTAall recombinant viruses infected PBMCs at 1dpi, 2dpi, 4dpi and 7dpi.

Jie Lu (139953)
Subhash C. Verma (198268)
Qiliang Cai (180057)
Abhik Saha (177355)
Richard Kuo Dzeng (335418)
Erle S. Robertson (177370)

February 2013

(A) Immunofluorescence assay for PBMCs infected by KSHV BAC36 wt, RTA1st and RTAa...

Real-Time quantitative PCR of purified transiently transfected plasmid DNA in HeLa S3 and SH-SY5Y cells.

Dan Y. Even (1805734)
Adi Kedmi (1805725)
Shani Basch-Barzilay (1805737)
Diana Ideses (1805743)
Ravid Tikotzki (1805746)
Hila Shir-Shapira (1805722)
Orit Shefi (699202)
Tamar Juven-Gershon (611679)

February 2016

HeLa S3 and SH-SY5Y cells were transiently transfected with pRc/CMV, natural CMV, SCP2 or SCP3 ve...

BV structural proteins incorporation and genome integrity analysis.

Shufen Li (746508)
Manli Wang (646363)
Shu Shen (746509)
Zhihong Hu (316172)
Hualin Wang (514306)
Fei Deng (170132)

May 2015

(A) Western blot analysis of structural proteins incorporated into recombinant BVs. Equal copy nu...

Transfection of Full length BAC GFP-EBV into 293T cells.

Sabyasachi Halder (356359)
Masanao Murakami (356361)
Subhash C. Verma (198268)
Pankaj Kumar (63234)
Fuming Yi (370254)
Erle S. Robertson (177370)

February 2013

(A) BAC GFP-EBV DNA was transfected to 293T and stable cell line generated as screened by puromyc...

Quantitation of viral DNA in transduced Vero cells.

Reuven Rasooly (394508)
Bradley Hernlem (442097)
Mendel Friedman (442098)

August 2013

Vero cells transduced with Ad-CMV-GFP were treated with 1 µM AFB1, 1% milk, or 1 ng/ml ricin. The...

Knockdown of MCM7 increases viral DNA replication.

Tamara Evans Braun (166141)
Emma Poole (166148)
John Sinclair (166153)

May 2012

HFFFs were transfected with siRNAs to MCM7 or a scramble control in the presence of serum. 48 hou...

Analysis of the replication capacity of the SIVFIV CA-p1-NC(1–9) chimera in permissive 293T cells by detecting the nuclear circular forms of unintegrated viral DNA.

María J. Esteva (668438)
José L. Affranchino (668439)
Silvia A. González (668440)

December 2014

(A) Left panel: Detection of late RT products at 48 h post-infection of 293T cells with VSV-G-pse...

Viral transduction efficiency determined via FACS and relative gene expression determined via quantitative real-time PCR.

Dong-Sik Ham (485770)
Juyoung Shin (485771)
Ji-Won Kim (174475)
Heon-Seok Park (485772)
Jae-Hyoung Cho (485773)
Kun-Ho Yoon (174472)

November 2013

(A) Various viral titers were tested using green fluorescent protein (GFP) as a reporter gene. Sa...

Knockdown of Cdc6 increases viral DNA replication.

Tamara Evans Braun (166141)
Emma Poole (166148)
John Sinclair (166153)

May 2012

HFFFs were transfected with siRNA Cdc6 or a scramble control in the presence of serum. 48 hours p...

Analysis of viral replication in Sf9 cells transfected/infected with AcMNPV recombinants with e25 under control of alternative promoters.

Xiao-Chun Luo (422940)
Shan-Shan Wang (233149)
Jie Zhang (64655)
Duo-Duo Qian (422941)
Si-Min Wang (422942)
Lu-Lin Li (422943)

June 2013

(A) Fluorescence microscopy of Sf9 cells transfected with vAcPH-gfp, vAce25ko-PH-...

Analysis of late gene expression in Sf9 cells transfected by recombinant AcMNPV bacmids with e25 deleted or under control of alternative promoters.

Xiao-Chun Luo (422940)
Shan-Shan Wang (233149)
Jie Zhang (64655)
Duo-Duo Qian (422941)
Si-Min Wang (422942)
Lu-Lin Li (422943)

June 2013

(A) Schematic maps of the structures of the recombinant AcMNPV bacmids with e25 mutants. A...

Characterization of recombinant bacmids and viruses.

Shufen Li (746508)
Manli Wang (646363)
Shu Shen (746509)
Zhihong Hu (316172)
Hualin Wang (514306)
Fei Deng (170132)

May 2015

(A) Construction of fp25k knockout bacmid. The fp25k gene in AcBacΔcc bacmid...

Virus growth and viral DNA replication analysis curves.

Huan Yu (140826)
Jiao Meng (482119)
Jian Xu (31545)
Tong-xian Liu (5664841)
Dun Wang (525747)

August 2015

A: Virus growth curves as determined by TCID50 endpoint dilution assays. HzAM1 cells w...

Viral DNA replication and gene transcription in cells transfected with vA4prm-VP39-EGFP bacmid DNA.

Jun Zhang (48506)
Xue-Mei Chen (130273)
Chun-Dong Zhang (629729)
Qian He (248656)
Zhan-Qi Dong (629730)
Ming-Ya Cao (629731)
Xiao-Long Dong (515229)
Cai-Xia Pan (515231)
Cheng Lu (57646)
Min-Hui Pan (515234)

September 2014

(A) Total cellular DNA was extracted from BmN-SWU1 and BmN-SWU2 cells at indicated times after vA...

Virus titer determination and qPCR analysis of BV genomic DNA copy number in the supernatant.

Shufen Li (746508)
Manli Wang (646363)
Shu Shen (746509)
Zhihong Hu (316172)
Hualin Wang (514306)
Fei Deng (170132)

May 2015

Sf9 cells were infected with vAcΔcc or vAcΔccΔfp25k at an MOI of 5. The supe...

Quantitative analysis of KSHV BAC36 wt, RTA1st and BAC RTAall recombinant viruses infected PBMCs at 1dpi, 2dpi, 4dpi and 7dpi.

Jie Lu (139953)
Subhash C. Verma (198268)
Qiliang Cai (180057)
Abhik Saha (177355)
Richard Kuo Dzeng (335418)
Erle S. Robertson (177370)

February 2013

(A) Immunofluorescence assay for PBMCs infected by KSHV BAC36 wt, RTA1st and RTAa...

Real-Time quantitative PCR of purified transiently transfected plasmid DNA in HeLa S3 and SH-SY5Y cells.

Dan Y. Even (1805734)
Adi Kedmi (1805725)
Shani Basch-Barzilay (1805737)
Diana Ideses (1805743)
Ravid Tikotzki (1805746)
Hila Shir-Shapira (1805722)
Orit Shefi (699202)
Tamar Juven-Gershon (611679)

February 2016

HeLa S3 and SH-SY5Y cells were transiently transfected with pRc/CMV, natural CMV, SCP2 or SCP3 ve...

BV structural proteins incorporation and genome integrity analysis.

Shufen Li (746508)
Manli Wang (646363)
Shu Shen (746509)
Zhihong Hu (316172)
Hualin Wang (514306)
Fei Deng (170132)

May 2015

(A) Western blot analysis of structural proteins incorporated into recombinant BVs. Equal copy nu...

Transfection of Full length BAC GFP-EBV into 293T cells.

Sabyasachi Halder (356359)
Masanao Murakami (356361)
Subhash C. Verma (198268)
Pankaj Kumar (63234)
Fuming Yi (370254)
Erle S. Robertson (177370)

February 2013

(A) BAC GFP-EBV DNA was transfected to 293T and stable cell line generated as screened by puromyc...

Quantitation of viral DNA in transduced Vero cells.

Reuven Rasooly (394508)
Bradley Hernlem (442097)
Mendel Friedman (442098)

August 2013

Vero cells transduced with Ad-CMV-GFP were treated with 1 µM AFB1, 1% milk, or 1 ng/ml ricin. The...

Knockdown of MCM7 increases viral DNA replication.

Tamara Evans Braun (166141)
Emma Poole (166148)
John Sinclair (166153)

May 2012

HFFFs were transfected with siRNAs to MCM7 or a scramble control in the presence of serum. 48 hou...

Analysis of the replication capacity of the SIVFIV CA-p1-NC(1–9) chimera in permissive 293T cells by detecting the nuclear circular forms of unintegrated viral DNA.

María J. Esteva (668438)
José L. Affranchino (668439)
Silvia A. González (668440)

December 2014

(A) Left panel: Detection of late RT products at 48 h post-infection of 293T cells with VSV-G-pse...

Viral transduction efficiency determined via FACS and relative gene expression determined via quantitative real-time PCR.

Dong-Sik Ham (485770)
Juyoung Shin (485771)
Ji-Won Kim (174475)
Heon-Seok Park (485772)
Jae-Hyoung Cho (485773)
Kun-Ho Yoon (174472)

November 2013

(A) Various viral titers were tested using green fluorescent protein (GFP) as a reporter gene. Sa...

Knockdown of Cdc6 increases viral DNA replication.

Tamara Evans Braun (166141)
Emma Poole (166148)
John Sinclair (166153)

May 2012

HFFFs were transfected with siRNA Cdc6 or a scramble control in the presence of serum. 48 hours p...

Quantitative-PCR analysis of viral DNA replication in Sf9 cells transfected by recombinant AcMNPV bacmids with <i>e25</i> deleted or under control of <i>ie1</i> promoter.

Abstract

Extracted data

Quantitative-PCR analysis of viral DNA replication in Sf9 cells transfected by recombinant AcMNPV bacmids with <i>e25</i> deleted or under control of <i>ie1</i> promoter.

Abstract

Extracted data

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