Upregulation of the level of expression of Kv4.1 subunits in Kv4.2−/− preoptic neurons.

<p>A. Single channel confocal images showing immunoreactivity to Kv4.1 (<i>Left</i>, green) and Kv4.2 (<i>Middle</i>, red) and their superimposition (<i>Right</i>, merge) obtained from dissociated w-t (upper row) and Kv4.2−/− (lower row) preoptic neurons. B. Cumulative histograms of the Kv4.1 staining intensity in w-t (blue circles, n = 708 neurons) and Kv4.2−/− neurons (red triangles, n = 811 neurons). The distributions were significantly different (P<0.01, Kolmogorov-Smirnov test). C. qPCR analysis of Kv4.1 (left) and Kv4.3 gene (right) expression in preoptic area from w-t and Kv4.2−/− mice (n = 6 mice each). For normalization GAPDH was selected as housekeeping gene. Bars represent averaged normalized concentrations ± SD. ** represents significant differences between w-t and Kv4.2−/− groups P<0.01 (t-test).