Lack of Kv1.2 and Kv2.2 colocalization at the AIS of cultured hippocampal neurons.

<p><u>A</u>–<u>B</u> and <u>C</u>–<u>D</u>: Pairs of representative confocal microscopy photomicrographs of hippocampal neurons cultured for 18 days, double immunostained for Kv1.2 (red) and Kv 2.2 (green), and counterstained with DAPI (<u>A</u>–<u>D</u>). Note that Kv1.2-expressing AISs (arrows) lack Kv2.2 immunostaining (Kv2.2) and vice versa (<u>E</u>). Histogram shows the proportion of neurons expressing Kv1.2, Kv2.2, neither or both at the same AIS at 18 DIV (the data represent the mean ± SE from three independent experiments). Scale bar = 25 µm.