Addition of E64 restores Δ<i>srv in vitro</i> biofilm formation to wild-type levels.

<p>E64 (100 µM) was added to each well at the time of seeding of wild-type and Δ<i>srv</i> RGAS053, MGAS315, and MGAS5005 24 h biofilms. E64 restored biofilm formation of all Δ<i>srv</i> strains to wild-type levels. RGAS053 biofilm formation was increased compared to untreated RGAS053. Each reported value for the CV assay is an average of 6 replicates and is adjusted by the dilution factor required to obtain a spectrophometric reading (OD_{600 nm}) (*<i>p</i>≤.01, **<i>p</i>≤0.001, ***<i>p</i>≤.0001; unpaired t-test).