Formation of biofilms by SCVs may involve extracellular proteins.

<p>The extent of biofilm production was measured by crystal violet staining (A_{560 nm}) of normal CF07-L and SCV CF07-S strains as a function of glucose (A) and NaCl (B) concentrations following a 48-h incubation period. Statistically significant differences between strains are indicated for each concentration of glucose and NaCl (<i>P</i><0.05; two-way ANOVA with Bonferonni's posttest, <i>n</i> = 3). (C) Susceptibility of SCVs' biofilms to treatment with proteinase K. Statistically significant differences between control and treated conditions are indicated (*, <i>P</i><0.05; **, <i>P</i><0.01; unpaired <i>t</i> test, <i>n</i> = 3). Results are normalized according to the control condition for each strain. (D) Expression ratio of the <i>icaC</i> gene as a function of growth for strains CF07-L, CF07-S and CF07-S in the presence of menadione, which restores normal growth. Results are expressed according to CF07-L in the early exponential phase of growth. No statistically significant difference was revealed between conditions for each growth phase (ANOVA with Dunnett's posttest, <i>n</i> = 3–4). Results are expressed as means with standard deviations.