Inactivation of <i>srv</i> in RGAS053 and MGAS315 resulted in decreased biofilm formation.

<p>Log-phase cultures of (A) RGAS053 (solid line) and RGAS053Δ<i>srv</i> (dashed line), (B) MGAS315 (solid line) and MGAS315Δ<i>srv</i> (dashed line), or (C) MGAS5005 (solid line) MGAS5005Δ<i>srv</i> (dashed line) were grown in 6-well plates and adherence was measured over a course of 48h using a CV staining assay. All Δ<i>srv</i> mutants were significantly reduced in forming biofilms compared to wild-type strains. Each reported value for the CV assay is an average of 6 replicates and is adjusted by the dilution factor required to obtain a spectrophometric reading (OD_{600 nm}) (*<i>p</i>≤0.01, **<i>p</i>≤0.001, ***<i>p</i>≤.0001; unpaired t-test).