<i>In-vitro</i> biofilm kinetic assay.

<p>Biofilm formation was assayed in 6 well polystyrene tissue culture plates. The wild type V288 and Δ<i>lytB</i> mutant GP1485 strains were inoculated from frozen stocks and incubated at 37°C in a CO₂ enriched atmosphere. At 2, 4, 6, 8, 10 and 24 hours of incubation, plates were washed and stained with crystal violet. Biofilm was removed with ethanol and quantified at 590 nm wavelength. Data represent the mean ± SEM of two experiments performed in triplicate. The asterisks indicate *<i>P</i> < 0.05.