Oxygen consumption.

<p>Control 1 (A) and NDUFS2 patient's (B) fibroblasts were grown in GLU or GAL medium and in GAL supplemented with 0.5 mM AICAR (GAL+AICAR) for 72 hrs. Media were changed to unbuffered media and oxygen consumption rates (OCR) were measured by an XF24 instrument. Basal OCR was measured (basal) before the addition of uncoupler and maximal rate (CCCP) after. The results are presented as rates subtracted by non mitochondrial oxygen consumption (in the presence of rotenone and antimycin) and normalized to cell amount measured by methylene blue (MB)at A₆₂₀, +/− standard deviation. *p<0.05.