Monitoring the oxygen consumption rate (OCR) in SCCVII and HT29 cells using XF96 Analyzer (Seahorse Bioscience).
- Yoichi Takakusagi (212143)
- Shingo Matsumoto (298080)
- Keita Saito (298078)
- Masayuki Matsuo (634288)
- Shun Kishimoto (634289)
- Jonathan W. Wojtkowiak (634290)
- William DeGraff (342312)
- Aparna H. Kesarwala (634291)
- Rajani Choudhuri (634292)
- Nallathamby Devasahayam (298084)
- Sankaran Subramanian (298086)
- Jeeva P. Munasinghe (298088)
- Robert J. Gillies (224878)
- James B. Mitchell (201891)
- Charles P. Hart (634293)
- Murali C. Krishna (298090)
Publication date
September 2014
DOIAbstract
<p><b>A, C</b>, OCR trace before and after the treatment with 0.2, 0.5 or 2 mM of exogenous pyruvate. <b>B,</b> OCR increase ratio (%) after the treatment of SCCVII and HT29 cells with each concentration of pyruvate. <b>C</b>, D, OCR response by the treatment of SCCVII with 2 mM pyruvate before (C) and after (D) incubating the inhibitors (antimycin A and rotenone) of mitochondrial respiratory chain.</p
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