Changes in the ratio of glycolysis and mitochondrial oxidation upon treatment with AICAR + MTX.

<p>Rate of mitochondrial oxygen consumption and glycolysis were assayed by Seahorse XF96 analyzer on day 1 and day 6 as described in Materials and Methods (n = 23/23/23/23). The OCR/ECAR ratio was depicted that reflects the ratio of mitochondrial oxidation and glycolytic flux. <i>OCR</i>-oxygen consumption rate; <i>ECAR</i>-extracellular acidification rate. A representative result is shown. *, ** and *** indicate statistically significant difference between control and treated cells at p<0.05, p<0.01 and p<0.001, respectively.