TGF-β1-mediated enhancement of mitochondrial respiration in NIH/3T3 cells.

<p><b>A</b>, Endogenous oxygen consumption rate (OCR) of naive (TGF-β1-untreated) and differentiated (TGF-β1-treated) NIH/3T3 cells measured using Seahorse XF-96. Basal respiration was inhibited with oligomycin (inhibitor of oxidative phosphorylation), followed by treatment with FCCP (uncoupler of mitochondrial oxidative phosphorylation) and antimycin A (inhibitor of mitochondrial respiratory chain). <b>B</b>, Quantification of OCR in naive and TGF-β1-treated NIH/3T3 cells at baseline and following treatment with oligomycin, FCCP and antimycin A. Shown are averages of at least three independent measurements ± standard error of mean. White circles and bars for naive, TGF-β1-untreated cells and black circles and bars for TGF-β1-treated cells.