Effect of maternal prenatal androgenization on hepatic metabolic and signaling pathways.

<p>A-B) Immunohistochemistry for PEPCK (brown) in adult liver sections. A) Hepatocytes are stained while supporting tissue, such as the capsule (arrow) are not immunostained (scale bar = 100 µm). B) Higher power section of the more intensely stained hepatocytes around the hepatic portal vessels (HPV). Arrow indicates no staining associated with the non-hepatocytes in this region (scale bar = 20 µm). Representative sections were selected from control animals. QRT-PCR showing the effect of maternal androgenization on hepatic <i>PEPCK</i> (C), <i>MAP2K4</i> (D), <i>UGCG</i> (E), <i>ACADM</i> (F), <i>IGF1</i> (G), <i>IGFBP1</i> (H) and <i>IGF1R</i> (I) mRNA expression. Values represent mean ± SEM and * = <i>P</i><0.05, ** = <i>P</i><0.01.