Expression of Fgf15 with or without N-terminal signal peptide in <i>E. coli</i>.

<p>M: protein molecular weight marker, lane 1: soluble lysate fraction from <i>E. coli</i> containing pET28a(+), lane 2: insoluble lysate fraction from <i>E. coli</i> containing pET28a(+), lane 3: soluble lysate fraction from <i>E. coli</i> containing pET/Fgf15, lane 4: insoluble lysate fraction from <i>E. coli</i> containing pET/Fgf15, lane 5: soluble lysate fraction from <i>E. coli</i> containing pET/tFgf15, lane 6: insoluble lysate fraction from <i>E. coli</i> containing pET/tFgf15.