SDS-PAGE of TAT-LUC protein.
- Tingyu Yu (4585810)
- Jiao Lin (3983147)
- Jin Zhao (94790)
- Wei Huang (36889)
- Lingwen Zeng (485807)
- Zhiyuan Fang (537089)
- Ning Xu (52890)
Publication date
November 2017
DOIAbstract
<p>(A) The protein was induced with IPTG (0.5 mM) at 22°C for 16 h in E. coli BL21(DE3) cells, and analyzed by 10% polyacrylamide SDS-PAGE. Lane 1, uninduced whole bacterial proteins; lane 2, supernatant of uninduced cell lysate; lane 3, pellet of uninduced cell lysate; lane 4, induced whole bacterial proteins; lane 5, supernatant of induced cell lysate; lane 6, pellet of induced cell lysate; Lane M, molecular weight marker. (B) Isolation of TAT-LUC. Lane 1, supernatant of cell lysate; Lane 2, purified TAT-LUC; lane M, molecular weight marker.</p
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