Lanes M: Protein markers; 1: Uninduced E. coli cells; 2: Lysate of the induced E. coli cells; 3: Soluble fraction of the cell lysate; 4: Insoluble fraction of the cell lysate.</p
<p>Total cell lysates without IPTG induction (N), the soluble (S) and insoluble fractions (P) from I...
<p>(A) Western blot analysis of the soluble (sup) and insoluble (ppt) fractions of <i>E. coli</i> ex...
<p>Recombinant proteins were expressed in <i>E. coli</i> BL21 (DE3) or SI and purified from the bact...
<p>(A) 10% SDS-PAGE analysis of the recombinant proteins from the culture medium (Med), periplasmic ...
<p>After induction with IPTG for 12 h, <i>E. coli</i> cells were harvested, total cell contents (W) ...
<p>The expression of recombinant fusion proteins were analyzed by SDS-PAGE. M, molecular size protei...
<p><b>Copyright information:</b></p><p>Taken from "Production of soluble mammalian proteins in Esche...
<p>Lanes: M, protein markers; 1, whole cell protein of pHsh transformed ...
<p>(A) SDS-PAGE analysis of insoluble (lanes 1, 3) and soluble (lanes 2, 4) fractions after cell lys...
<p>Each fusion protein was expressed in <i>E. coli</i> and analyzed by SDS-PAGE followed by Coomassi...
<p>(A) The number of Buf IIIb-L genes cloned in pLpp-OmpA-B<sub>n</sub> was determined by digesting ...
<p>GST and GST-ST1, -ST2 and -ST3 fusions proteins were expressed in <i>E.coli</i>, extracted, and r...
<p>Protein samples were separated by 15% SDS-PAGE and stained with Coomassie brilliant blue. Lane 1,...
<p>SDS-PAGE analysis of ONC-(P)GKY20 and ONC-DCless-H6-(P)GKY20 fusion proteins. Lane M1: marker (14...
<p>(A) SDS-PAGE of the whole <i>E. coli</i> cell extract after 3 h of induction of both constructs. ...
<p>Total cell lysates without IPTG induction (N), the soluble (S) and insoluble fractions (P) from I...
<p>(A) Western blot analysis of the soluble (sup) and insoluble (ppt) fractions of <i>E. coli</i> ex...
<p>Recombinant proteins were expressed in <i>E. coli</i> BL21 (DE3) or SI and purified from the bact...
<p>(A) 10% SDS-PAGE analysis of the recombinant proteins from the culture medium (Med), periplasmic ...
<p>After induction with IPTG for 12 h, <i>E. coli</i> cells were harvested, total cell contents (W) ...
<p>The expression of recombinant fusion proteins were analyzed by SDS-PAGE. M, molecular size protei...
<p><b>Copyright information:</b></p><p>Taken from "Production of soluble mammalian proteins in Esche...
<p>Lanes: M, protein markers; 1, whole cell protein of pHsh transformed ...
<p>(A) SDS-PAGE analysis of insoluble (lanes 1, 3) and soluble (lanes 2, 4) fractions after cell lys...
<p>Each fusion protein was expressed in <i>E. coli</i> and analyzed by SDS-PAGE followed by Coomassi...
<p>(A) The number of Buf IIIb-L genes cloned in pLpp-OmpA-B<sub>n</sub> was determined by digesting ...
<p>GST and GST-ST1, -ST2 and -ST3 fusions proteins were expressed in <i>E.coli</i>, extracted, and r...
<p>Protein samples were separated by 15% SDS-PAGE and stained with Coomassie brilliant blue. Lane 1,...
<p>SDS-PAGE analysis of ONC-(P)GKY20 and ONC-DCless-H6-(P)GKY20 fusion proteins. Lane M1: marker (14...
<p>(A) SDS-PAGE of the whole <i>E. coli</i> cell extract after 3 h of induction of both constructs. ...
<p>Total cell lysates without IPTG induction (N), the soluble (S) and insoluble fractions (P) from I...
<p>(A) Western blot analysis of the soluble (sup) and insoluble (ppt) fractions of <i>E. coli</i> ex...
<p>Recombinant proteins were expressed in <i>E. coli</i> BL21 (DE3) or SI and purified from the bact...
DOI
Add to ORKG