Expanded CD34⁺ CD133⁺ cells give robust reconstitution in both neonate and adult recipients.

<p>(A) Comparison of reconstitution levels in mice injected with different numbers of expanded cells. Sub-lethally irradiated adult NSG recipients were injected with cultured cells containing 0.5×10⁵, 2.5×10⁵ or 5×10⁵ CD34⁺ CD133⁺ cells. Eight weeks later, PBMCs were analyzed for human and mouse CD45 expression. Mean ± SD is shown, n = 6–8 per group. (B) Comparison of reconstitution levels in the bone marrow of neonate and adult recipients. Sub-lethally irradiated adult NSG recipients were injected with expanded cells containing 2.5×10⁵ CD34⁺ CD133⁺ cells. Sub-lethally irradiated neonates were injected with 1×10⁵ unexpanded or expanded CD34⁺ CD133⁺ cells. Twelve to sixteen week later, bone marrow was analyzed for human and mouse CD45 expression. Mean ± SD is shown, n = 4–6 per group.