Comparison of NSG mouse engraftment with human cord blood cells expanded with Tat-MYC/Tat-Bcl-2 or fresh cord blood.

<p><b>A.</b> hCD45⁺ cell staining in the BM of sublethally irradiated NSG mice were given transplants of 10⁶ cord blood cells expanded <i>ex vivo</i> in a cocktail of cytokines (first panel), expanded in a cocktail of cytokines supplemented with Tat-MYC/Tat-Bcl-2 (second panel), or 1×10⁷ fresh unmanipulated cord blood cells (third panel). <b>B.</b> A dot plot showing BM cells from a cohort of NSG xenochimaeric mice transplanted of 10⁶ cord blood cells expanded <i>ex vivo</i> in a cocktail of cytokines (black circles), expanded in a cocktail of cytokines supplemented with Tat-MYC/Tat-Bcl-2 (open squares), or 1×10⁷ fresh unmanipulated cord blood cells (black diamonds) were stained for human CD45. <b>C.</b> A dot plot shown human CD34⁺/CD38⁺ cells in the bone marrow 3/5 mice transplanted with cord blood expanded in cytokines alone (black circles), CD34⁺/CD38⁺ cells in the bone marrow of 5/5 mice transplanted with cord blood expanded in cytokines plus Tat-MYC/Tat-Bcl-2 (open squares), and CD34⁺/CD38⁺ cells in the bone marrow of 1 out of 3 mice transplanted with unmanipulated cord blood (black diamonds).