Comparison of NSG mouse engraftment with CD34⁺ cells isolated from human cord blood cells after expansion with Tat-MYC, Tat-Bcl-2, Tat-MYC/Tat-Bcl-2 or Tat-Cre.

<p><b>A.</b> Cohorts of irradiated NSG mice were given transplants of 10⁵ CD34⁺ cells isolated from CB expanded <i>ex vivo</i> in CB media supplemented with Tat-MYC/Tat-Bcl-2. The NSG xenochimaeric mice generated with isolated CD34⁺ cells were euthanized and used to collect bone marrow cells. The BM cells were stained for human hCD45 (1^st panel). Gating on CD45⁺ cells, we observed human CD34⁺/CD38⁺ cells (2^nd panel), CD19⁺ cells (3^rd panel) and CD11b⁺/CD33⁺ cells (4^th panel) in the bone marrow. <b>B.</b> A dot plot representation of the percent CD45⁺ engraftment in the BM of mice transplanted of 10⁵ CD34⁺ cells isolated from CB expanded <i>ex vivo</i> in CB media supplemented with Tat-MYC (black circles), Tat-Bcl-2 (open squares), Tat-MYC/Tat-Bcl-2 (black diamonds) or Tat-Cre (black X). <b>C</b> A dot plot showing the percent of CD45⁺ cells shown in B that stain positive for CD34. <b>D and E.</b> A dot plot showing the lineage positive engraftment in the BM of mice shown in B.