Bone marrow extracts enhance human bone marrow derived CD34 positive cells engraftment in immunodeficient mice.

<p>Two groups of NSG mice (n = 5 each) received 50×10³ bone marrow derived CD34 positive cells previously cultured for 13 days, in the presence or absence of BME. A third group (n = 5) served as a negative control and was injected with saline. Mice were sacrificed six weeks after transplantation. <b>A</b>) The percentage of engraftment was determined using the human pan leukocyte CD45 marker. <b>B</b>) The contribution of myeloid, lymphoid and erythroid populations to total human leukocyte engraftment was determined using CD33, CD19, CD36 and CD45 markers, respectively. *: p<0.05.