Increased engraftment of CD34+GFP+ cells with NA10HD.

<p>CD45+CD34+GFP+ cells recovered from bone marrow harvested from NSG mice 12–15 week post transplantation with lentiviral transduced CD34+ cells that remained in culture for 24 hours (“Unexpanded”) after transduction (a) or expanded for 1 week (“Expanded”) post-transduction prior to transplant (b). Statistical comparisons performed using Mann-Whitney U testing with a p-value of 0.05 being considered significant. In the unexpanded group there was a higher percentage of CD45+CD34+GFP+ cells in the NA10HD group compared to MSCV GFP that trended toward but did not meet statistical significance (2.4 vs 1.7%, p = 0.06, n = 30 mice per condition), the Expanded group showed a higher CD45+CD34+GFP+ percentage for NA10HD (6.96 vs 2.8%, p = 0.006, n = 26 mice per condition).