Effects of METH on GLUT3 expression in human neurons:

<p>Immunocytochemistry of GLUT3 (red) merged with NF (green) and DAPI (blue) in control (<b>A–B</b>), 20 µM METH (<b>C–D</b>), and 100 µM METH+100 µM ALC (<b>E–F</b>). Rabbit anti-GLUT3 and mouse anti-NF were used as primary antibodies for overnight at 4°C. Anti-rabbit-IgG Alexa Fluor 594 for GLUT3 and anti-mouse-IgG Alexa Fluor 488 for NF were used as secondary antibodies. Scale bar indicates 10 µm in all panels.