Cytokine profile of CD4⁺ and CD8⁺ T cells after SLA stimulation.

<p>Purified CD4⁺ and CD8⁺ T cells were adjusted to 1–2×10⁵ cells/well in a U-bottomed 96-well plates and co-cultured with 1∶10 of mitomycin treated autologous monocytes in cRPMI 1640 supplemented with 10% human AB Rh+ serum. IFN-γ, IL-5, IL-10, and IL-13 were titrated on supernatant of SLA stimulated of CD4⁺ (A) and CD8⁺ (B) T cells at 72 hrs of culture using sandwich ELISA method. The amount of IL-5 was not detectable in the culture supernatants. Filled symbols represent HCL volunteers; Open symbols represent healthy controls.