Analysis of the cytokine responses after challenge with <i>L. major</i>.

<p>As in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067453#pone-0067453-g005" target="_blank">Figure 5</a>, but 12 weeks after infection with <i>L. major</i>, total cell suspensions of ear-infected draining LN were restimulated <i>in vitro</i> for 72 h with 10 µg/ml of SLA. The levels of IFN-γ, IL-4, and IL-10 cytokines in the culture supernatants were determined by ELISA. Data is shown as the mean ± SEM of the ratio of (A) IFN-γ/IL-4 and (B) IL-10 (n = 4–10).