LT expression on human immune cell populations.

<p>Human CD4⁺ T cell expression of LT. <b>A</b>. Sorted CD4⁺ T cells were labeled with CFSE and proliferation and LT expression was monitored at indicated time points following stimulation with anti-CD3 and anti-CD28. <b>B</b>, <b>C</b>. Co-expression of LT with T cell activation markers CD25 (<b>B</b>) or CD45RO (<b>C</b>) on CD4⁺ gated cells. <b>D</b>, <b>E</b>. CD8⁺ T cell expression of LT. Co-expression of LT with CD25 (<b>D</b>) or CD45RO (<b>E</b>) on CD8⁺ gated cells. For CD4⁺ and CD8⁺ T cell activation, PBMCs were stimulated with anti-CD3 and anti-CD28 mAbs for 2 days. <b>F</b>. Human B cell expression of LT. B cells were stimulated with anti-IgM and BAFF for two days, then LT expression determined on CD19⁺ B cells with CD69 as a marker for activation. <b>G</b>. Human monocyte expression of LT. CD14⁺ monocytes were stimulated with LPS, with activation status assessed on the basis of HLA-DR up-regulation.