Surface expression of CD4 and different receptors at different time points.

<p>Purified PBLs were stimulated for 12 days with CD3/CD28 beads. After 12 days, beads were removed and the cells set up in new media. Cytokines were added in the following concentrations IL-12: 4 ng/mL, IL-15: 10 ng/mL, IL-18: 40 ng/mL, TL1A: 100 ng/mL, TL1A Ab: 1 µg/mL. Surface expression of co-receptors and activation markers CD25, CD134, CD154 and LFA-1 after 72 h. Upper panels: Gating strategy for lymphocytes. Lower panels: Co-staining for CD4 and CD134, CD154, CD25 of LFA-1, % positive cells and MFI for upper right quadrant are shown. Data are representative of results obtained with cells from three different donors.