Additional file1: of Finite-size effects in transcript sequencing count distribution: its power-law correction necessarily precedes downstream normalization and comparative analysis

Figure S1. Concentration versus total mapped reads of the dilution data set. Figures S1A to D shows the concentrations of the AGS cell line (12pM, 3pM) and NUGC3 cell line (12pM, 6pM, 3pM, 1.5pM) versus the respective total mapped reads by the 4 mapping methods: Bowtie1, Bowtie2(global), Novoalign and BWA. Regardless of the mapping methods, the sequencing depth (i.e., the total mapped reads) is shown to be linearly proportional to the system size (in terms of transcript concentration) in the logarithmic scale. Overall, the dilution data set attempts to mimic a system of various sizes of finite-size effects. (PNG 371 kb