Probit regression analysis of data set of 10 runs of Fno-RPA and qPCR using Minitab®.

Sensitivity analysis of the exo-RPA assay.

Jianchang Wang (3612506)
Libing Liu (1401136)
Jinfeng Wang (38441)
Xiaoxia Sun (684999)
Wanzhe Yuan (3612509)

January 2017

Different copy numbers of plasmid pMD19-T-TK DNA (106 to 100 copies) were a...

Fixation introduces a bias towards small RNA species in qRT-PCR.

Karl Kashofer (429432)
Christian Viertler (339303)
Martin Pichler (283174)
Kurt Zatloukal (234954)

July 2013

(A) Small RNA fragments like 5 s rRNA (∼120 bp) are present in cryopreserved (left) and fixed (ri...

Limit of detection between the RT-qPCR (Ct value), RT-LAMP (Tp value) and RT-LAMP LFD assays.

Ryan A. Waters (622968)
Veronica L. Fowler (622969)
Bryony Armson (622970)
Noel Nelson (622971)
John Gloster (622972)
David J. Paton (272094)
Donald P. King (116717)

August 2014

(a) RT-qPCR amplification Ct values corresponding to each of the 10-fold dilutions of RNA standar...

Probit regression analyses of RT-RPA assays using two primer combinations.

Pranav Patel (415128)
Ahmed Abd El Wahed (448246)
Oumar Faye (32488)
Pauline Prüger (3184779)
Marco Kaiser (240441)
Sasikanya Thaloengsok (755315)
Sukathida Ubol (171369)
Anavaj Sakuntabhai (50927)
Isabelle Leparc-Goffart (213294)
Frank T. Hufert (376414)
Amadou A. Sall (116184)
Manfred Weidmann (448254)
Matthias Niedrig (237181)

September 2016

(A) RF+RR3 and (B) RF2+RR2. The data sets of eight RT-RPA assay runs using serial dilutions of <i...

Performance and analytical sensitivity of the FMDV RT-RPA assay.

Ahmed Abd El Wahed (448246)
Ayman El-Deeb (448247)
Mohamed El-Tholoth (448248)
Hanaa Abd El Kader (448249)
Abeer Ahmed (448250)
Sayed Hassan (448251)
Bernd Hoffmann (191620)
Bernd Haas (448252)
Mohamed A. Shalaby (448253)
Frank T. Hufert (376414)
Manfred Weidmann (448254)

August 2013

A: Semi-logarithmic regression of the data collected from eight FMDV RT-RPA test runs on the RNA ...

MOESM5 of Ribosomal and non-ribosomal PCR targets for the detection of low-density and mixed malaria infections

Lara Amaral (3933215)
Daniela Robortella (6652802)
Luiz Guimarães (6652805)
Jean Limongi (6652808)
Cor Fontes (3414815)
Dhelio Pereira (510037)
Cristiana Brito (3741403)
Flora Kano (6652811)
Taís Sousa (3933221)
Luzia Carvalho (3417875)

April 2019

Additional file 5. Limit of detection (LOD) for (A) Pvr47 and (B) Pfr364 targets amplified by NR-qPC...

Probit regression analysis determining the limit of detection (the lowest viral load detectable in 95% of the time, corresponding to probit value 6.65) of Bam-W repeated- and LMP2 single- sequence qPCRs.

Armen Sanosyan (4398160)
Alexis Fayd’herbe de Maudave (4398166)
Karine Bollore (4398163)
Valérie Zimmermann (4398157)
Vincent Foulongne (156996)
Philippe Van de Perre (291400)
Edouard Tuaillon (81329)

August 2017

A series of 7 and 6 EBV concentrations for Bam-W and LMP2 qPCRs, respectively, were prepared by s...

Evaluation of the detection limit of RPA-nfo and RPA-exo using genomic DNA*.

Chien-Chung Chao (767988)
Tatyana Belinskaya (767989)
Zhiwen Zhang (767990)
Wei-Mei Ching (454580)

July 2015

*. Percent positive is calculated based on [# of positive/total # of samples tested]. For each sa...

qPCR assay performance on plasmid DNA.

Andrew Garcia (2268778)
Gargi Roy (4386271)
Christine Kiefer (675617)
Susan Wilson (134263)
Marcello Marelli (759717)

May 2019

The tRNApyl and pylRSwt qPCR assays were tested with a tenfold serial dilution of their respective p...

DNA detectability half-life of the different body parts tested calculated with probit models, and 83% fiducial confidence limits calculated to compared half-lives.

Nuria Macías-Hernández (3318675)
Kacie Athey (3743758)
Vanina Tonzo (5317718)
Owen S. Wangensteen (134811)
Miquel Arnedo (3243576)
James D. Harwood (2882087)

May 2018

DNA detectability half-life of the different body parts tested calculated with probit models, and...

Limit of detection of the assay for different HIV-1 subtypes as determined using probit regression analysis.

Francisco Martin (26407)
Claudia Palladino (500189)
Rita Mateus (111110)
Anna Bolzan (4252837)
Perpétua Gomes (257023)
José Brito (467452)
Ana Patrícia Carvalho (4252840)
Yolanda Cardoso (4252828)
Cristovão Domingos (4252834)
Vanda Sofia Lôa Clemente (4252831)
Nuno Taveira (232622)

July 2017

Limit of detection of the assay for different HIV-1 subtypes as determined using probit regressio...

Cross-validation of DNA samples quantification by qPCR.

Michele Simbolo (418972)
Marisa Gottardi (418973)
Vincenzo Corbo (242342)
Matteo Fassan (221852)
Andrea Mafficini (418974)
Giorgio Malpeli (324076)
Rita T. Lawlor (418975)
Aldo Scarpa (242345)

June 2013

Bland-Altman plots for inter-technology (NanoDrop or Qubit vs. qPCR) comparison of all samples (A...

Amplification curves in Mu RPA (A) and BU real-time PCR (B) assays applying serial dilutions (106–1) of the molecular DNA standard.

Michael Frimpong (550233)
Hubert Senanu Ahor (6288446)
Ahmed Abd El Wahed (448246)
Bernadette Agbavor (6288449)
Francisca Naana Sarpong (6288452)
Kenneth Laing (6288455)
Mark Wansbrough-Jones (347902)
Richard Odame Phillips (139062)

February 2019

No fluorescent signals were detected in RPA before four minutes because the strip was taken out for ...

FPKM quantitation of minicircle steady state transcripts.

Larry Simpson (774451)
Stephen M. Douglass (774452)
James A. Lake (774453)
Matteo Pellegrini (2078)
Feng Li (30515)

July 2015

Percent expression of steady state gRNA transcripts compared to percent of UC minicircle DNA per ...

Probit regression analysis to determine the limit of detection of the in-house HIV viral load assay.

Palvinder Kaur (531670)
Wei Xin Khong (334800)
Sue Yuen Wee (531671)
Eng Lee Tan (167237)
Juergen Pipper (30677)
Evelyn Koay (531672)
Kah Ying Ng (491452)
Joe Kwan Yap (491455)
Kuan Kiat Chew (491453)
Mei Ting Tan (491456)
Yee Sin Leo (134858)
Masafumi Inoue (521301)
Oon Tek Ng (134833)

March 2014

125.5 copies/mL (2.10 log10 copies/mL) at 95% CI (range 89.6–243.9 copies/mL; 1.95–2.3...

Sensitivity analysis of the exo-RPA assay.

Jianchang Wang (3612506)
Libing Liu (1401136)
Jinfeng Wang (38441)
Xiaoxia Sun (684999)
Wanzhe Yuan (3612509)

January 2017

Different copy numbers of plasmid pMD19-T-TK DNA (106 to 100 copies) were a...

Fixation introduces a bias towards small RNA species in qRT-PCR.

Karl Kashofer (429432)
Christian Viertler (339303)
Martin Pichler (283174)
Kurt Zatloukal (234954)

July 2013

(A) Small RNA fragments like 5 s rRNA (∼120 bp) are present in cryopreserved (left) and fixed (ri...

Limit of detection between the RT-qPCR (Ct value), RT-LAMP (Tp value) and RT-LAMP LFD assays.

Ryan A. Waters (622968)
Veronica L. Fowler (622969)
Bryony Armson (622970)
Noel Nelson (622971)
John Gloster (622972)
David J. Paton (272094)
Donald P. King (116717)

August 2014

(a) RT-qPCR amplification Ct values corresponding to each of the 10-fold dilutions of RNA standar...

Probit regression analyses of RT-RPA assays using two primer combinations.

Pranav Patel (415128)
Ahmed Abd El Wahed (448246)
Oumar Faye (32488)
Pauline Prüger (3184779)
Marco Kaiser (240441)
Sasikanya Thaloengsok (755315)
Sukathida Ubol (171369)
Anavaj Sakuntabhai (50927)
Isabelle Leparc-Goffart (213294)
Frank T. Hufert (376414)
Amadou A. Sall (116184)
Manfred Weidmann (448254)
Matthias Niedrig (237181)

September 2016

(A) RF+RR3 and (B) RF2+RR2. The data sets of eight RT-RPA assay runs using serial dilutions of <i...

Performance and analytical sensitivity of the FMDV RT-RPA assay.

Ahmed Abd El Wahed (448246)
Ayman El-Deeb (448247)
Mohamed El-Tholoth (448248)
Hanaa Abd El Kader (448249)
Abeer Ahmed (448250)
Sayed Hassan (448251)
Bernd Hoffmann (191620)
Bernd Haas (448252)
Mohamed A. Shalaby (448253)
Frank T. Hufert (376414)
Manfred Weidmann (448254)

August 2013

A: Semi-logarithmic regression of the data collected from eight FMDV RT-RPA test runs on the RNA ...

MOESM5 of Ribosomal and non-ribosomal PCR targets for the detection of low-density and mixed malaria infections

Lara Amaral (3933215)
Daniela Robortella (6652802)
Luiz Guimarães (6652805)
Jean Limongi (6652808)
Cor Fontes (3414815)
Dhelio Pereira (510037)
Cristiana Brito (3741403)
Flora Kano (6652811)
Taís Sousa (3933221)
Luzia Carvalho (3417875)

April 2019

Additional file 5. Limit of detection (LOD) for (A) Pvr47 and (B) Pfr364 targets amplified by NR-qPC...

Probit regression analysis determining the limit of detection (the lowest viral load detectable in 95% of the time, corresponding to probit value 6.65) of Bam-W repeated- and LMP2 single- sequence qPCRs.

Armen Sanosyan (4398160)
Alexis Fayd’herbe de Maudave (4398166)
Karine Bollore (4398163)
Valérie Zimmermann (4398157)
Vincent Foulongne (156996)
Philippe Van de Perre (291400)
Edouard Tuaillon (81329)

August 2017

A series of 7 and 6 EBV concentrations for Bam-W and LMP2 qPCRs, respectively, were prepared by s...

Evaluation of the detection limit of RPA-nfo and RPA-exo using genomic DNA*.

Chien-Chung Chao (767988)
Tatyana Belinskaya (767989)
Zhiwen Zhang (767990)
Wei-Mei Ching (454580)

July 2015

*. Percent positive is calculated based on [# of positive/total # of samples tested]. For each sa...

qPCR assay performance on plasmid DNA.

Andrew Garcia (2268778)
Gargi Roy (4386271)
Christine Kiefer (675617)
Susan Wilson (134263)
Marcello Marelli (759717)

May 2019

The tRNApyl and pylRSwt qPCR assays were tested with a tenfold serial dilution of their respective p...

DNA detectability half-life of the different body parts tested calculated with probit models, and 83% fiducial confidence limits calculated to compared half-lives.

Nuria Macías-Hernández (3318675)
Kacie Athey (3743758)
Vanina Tonzo (5317718)
Owen S. Wangensteen (134811)
Miquel Arnedo (3243576)
James D. Harwood (2882087)

May 2018

DNA detectability half-life of the different body parts tested calculated with probit models, and...

Limit of detection of the assay for different HIV-1 subtypes as determined using probit regression analysis.

Francisco Martin (26407)
Claudia Palladino (500189)
Rita Mateus (111110)
Anna Bolzan (4252837)
Perpétua Gomes (257023)
José Brito (467452)
Ana Patrícia Carvalho (4252840)
Yolanda Cardoso (4252828)
Cristovão Domingos (4252834)
Vanda Sofia Lôa Clemente (4252831)
Nuno Taveira (232622)

July 2017

Limit of detection of the assay for different HIV-1 subtypes as determined using probit regressio...

Cross-validation of DNA samples quantification by qPCR.

Michele Simbolo (418972)
Marisa Gottardi (418973)
Vincenzo Corbo (242342)
Matteo Fassan (221852)
Andrea Mafficini (418974)
Giorgio Malpeli (324076)
Rita T. Lawlor (418975)
Aldo Scarpa (242345)

June 2013

Bland-Altman plots for inter-technology (NanoDrop or Qubit vs. qPCR) comparison of all samples (A...

Amplification curves in Mu RPA (A) and BU real-time PCR (B) assays applying serial dilutions (106–1) of the molecular DNA standard.

Michael Frimpong (550233)
Hubert Senanu Ahor (6288446)
Ahmed Abd El Wahed (448246)
Bernadette Agbavor (6288449)
Francisca Naana Sarpong (6288452)
Kenneth Laing (6288455)
Mark Wansbrough-Jones (347902)
Richard Odame Phillips (139062)

February 2019

No fluorescent signals were detected in RPA before four minutes because the strip was taken out for ...

FPKM quantitation of minicircle steady state transcripts.

Larry Simpson (774451)
Stephen M. Douglass (774452)
James A. Lake (774453)
Matteo Pellegrini (2078)
Feng Li (30515)

July 2015

Percent expression of steady state gRNA transcripts compared to percent of UC minicircle DNA per ...

Probit regression analysis to determine the limit of detection of the in-house HIV viral load assay.

Palvinder Kaur (531670)
Wei Xin Khong (334800)
Sue Yuen Wee (531671)
Eng Lee Tan (167237)
Juergen Pipper (30677)
Evelyn Koay (531672)
Kah Ying Ng (491452)
Joe Kwan Yap (491455)
Kuan Kiat Chew (491453)
Mei Ting Tan (491456)
Yee Sin Leo (134858)
Masafumi Inoue (521301)
Oon Tek Ng (134833)

March 2014

125.5 copies/mL (2.10 log10 copies/mL) at 95% CI (range 89.6–243.9 copies/mL; 1.95–2.3...

Sensitivity analysis of the exo-RPA assay.

Jianchang Wang (3612506)
Libing Liu (1401136)
Jinfeng Wang (38441)
Xiaoxia Sun (684999)
Wanzhe Yuan (3612509)

January 2017

Different copy numbers of plasmid pMD19-T-TK DNA (106 to 100 copies) were a...

Fixation introduces a bias towards small RNA species in qRT-PCR.

Karl Kashofer (429432)
Christian Viertler (339303)
Martin Pichler (283174)
Kurt Zatloukal (234954)

July 2013

(A) Small RNA fragments like 5 s rRNA (∼120 bp) are present in cryopreserved (left) and fixed (ri...

Limit of detection between the RT-qPCR (Ct value), RT-LAMP (Tp value) and RT-LAMP LFD assays.

Ryan A. Waters (622968)
Veronica L. Fowler (622969)
Bryony Armson (622970)
Noel Nelson (622971)
John Gloster (622972)
David J. Paton (272094)
Donald P. King (116717)

August 2014

(a) RT-qPCR amplification Ct values corresponding to each of the 10-fold dilutions of RNA standar...

Probit regression analysis of data set of 10 runs of <i>Fno</i>-RPA and qPCR using Minitab<sup>®</sup>.

Abstract

Extracted data

Probit regression analysis of data set of 10 runs of <i>Fno</i>-RPA and qPCR using Minitab<sup>®</sup>.

Abstract

Extracted data

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