No fluorescent signals were detected in RPA before four minutes because the strip was taken out for mixing. The lowest number of copies detected for RPA and real-time PCR was 10 and 1 respectively.</p
An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that...
<p>The DNA of each H37Rv:XDR-TB mixture was diluted 10 fold serially and subjected to real-time PCR ...
<p><b>A</b>) Melting curve of the 11 reference genes showing a single pick (each including three tec...
A: Threshold time (Y-axis) was plotted versus the log10 concentration of molecules (X-axis) for the ...
<p>Different copy numbers of plasmid pMD19-T-TK DNA (10<sup>6</sup> to 10<sup>0</sup> copies) were a...
(A) NADH dehydrogenase 1 complementary DNA was serially (1:10) diluted to prepare a series of standa...
<p>A: Amplification curves. Ten-fold dilutions of standard DNA ranging from 10<sup>7</sup> copies/µL...
<p>Serial 10-fold dilutions of standard DEV DNA from 5.25×10<sup>10</sup> to 10<sup>2</sup> copies w...
<p>Amplification plots of real-time measurements for extracted RNA from 10-fold serial dilutions of ...
High and comparable efficiency values are the key for reliable quantification of target genes from e...
(A) Real-time R-CDA of different groups for R. raoultii DNA (each set 2 positive reactions and 2 neg...
<p>(A) Typical amplification curves of ten-fold serially diluted red fox DNA. (B) The standard curve...
<p>Real-time PCR standard curves showing the linear relationship between the log of known DNA concen...
<p>Figure <b>2a</b> shows the real time detection of IS<i>1081</i> amplification from a dilution ser...
<p style="text-align: justify;">Fluorescent monitoring of DNA amplification is the basis of real-tim...
An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that...
<p>The DNA of each H37Rv:XDR-TB mixture was diluted 10 fold serially and subjected to real-time PCR ...
<p><b>A</b>) Melting curve of the 11 reference genes showing a single pick (each including three tec...
A: Threshold time (Y-axis) was plotted versus the log10 concentration of molecules (X-axis) for the ...
<p>Different copy numbers of plasmid pMD19-T-TK DNA (10<sup>6</sup> to 10<sup>0</sup> copies) were a...
(A) NADH dehydrogenase 1 complementary DNA was serially (1:10) diluted to prepare a series of standa...
<p>A: Amplification curves. Ten-fold dilutions of standard DNA ranging from 10<sup>7</sup> copies/µL...
<p>Serial 10-fold dilutions of standard DEV DNA from 5.25×10<sup>10</sup> to 10<sup>2</sup> copies w...
<p>Amplification plots of real-time measurements for extracted RNA from 10-fold serial dilutions of ...
High and comparable efficiency values are the key for reliable quantification of target genes from e...
(A) Real-time R-CDA of different groups for R. raoultii DNA (each set 2 positive reactions and 2 neg...
<p>(A) Typical amplification curves of ten-fold serially diluted red fox DNA. (B) The standard curve...
<p>Real-time PCR standard curves showing the linear relationship between the log of known DNA concen...
<p>Figure <b>2a</b> shows the real time detection of IS<i>1081</i> amplification from a dilution ser...
<p style="text-align: justify;">Fluorescent monitoring of DNA amplification is the basis of real-tim...
An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that...
<p>The DNA of each H37Rv:XDR-TB mixture was diluted 10 fold serially and subjected to real-time PCR ...
<p><b>A</b>) Melting curve of the 11 reference genes showing a single pick (each including three tec...