NFκB has a greater upregulatory effect on the 5A allele than the 6A allele.

<p>RAW264 cells were transfected with an <i>MMP3</i> 5A-firefly luciferase reporter plasmid or an <i>MMP3</i> 6A-firefly luciferase reporter plasmid, and the pRL-TK plasmid (expressing <i>renilla</i> luciferase) to serve as a reference for transfection efficiency, together with a plasmid expressing p50 and/or a plasmid expressing p65. Luciferase reporter assay was performed using a dual-luciferase reporter assay system. Data shown are mean (± standard error of mean) of the ratio of firefly luciferase activity over <i>renilla</i> luciferase activity from four independent experiments. In panel (A), * indicates p = 0.07 comparing 5A and 5A plus p50. In panel (B), ** indicates p<0.01 comparing 5A and 5A plus p65. In panel (C), * denotes p<0.05 comparing 5A with 5A plus p50 and p65.