Comparison of relative luciferase activity between pGL3-basic-NOX4 (C) and pGL3-basic-NOX4 (T) plasmids.

<p>PBMC were transfected with C- or T-bearing reporter vectors with or without pretreatment of over-expression HNF3γ plasmid. Left panel: Bar chart of alterations in relative luciferase activity. Right panel: Representative western blot (top) and bar chart (below) of alterations in HNF3γ protein levels either pretreated with over-expression HNF3γ plasmid or not. Renilla luciferase activity was measured and normalized to firefly luciferase. Data represent mean ± SD of at least three independent experiments. *<i>P</i><0.05 <i>vs</i>. cells treated with either pGL3-basic-NOX4 (C) or pGL3-basic-NOX4 (T); ▲ <i>P</i><0.05 <i>vs</i>. cells treated with over-expression HNF3γ plasmid plus pGL3-basic-NOX4 (T).