PMS1077 inhibited TNF-α induced expression of NF-κB regulated reporter gene.

<p>HEK293T cells were transiently co-transfected with NF-κB-Firefly luciferase and TK-Renilla Luciferase reporter vectors for NF-κB activity assay. (A) NF-κB-dependent reporter gene expression induced by TNF-α. Transfected cells were treated with vehicle or TNF-α (1, 5, 10, 20 and 50 ng/ml) for 12 h; (B) TPCA-1 inhibited the expression of the TNF-α induced NF-κB dependent reporter gene. Transfected cells were treated with vehicle or TPCA-1(1, 2, 3, and 4 µM), then incubated with TNFα (20 ng/ml) for 12 h; (C) PMS1077 inhibited TNF-α induced, NF-κB dependent reporter gene expression. Transfected cells were treated with vehicle or PMS1077 (20, 40, 60 and 80 µM), then incubated with TNF-α (20 ng/ml) for 12 h; (D) PMS601 showed no effect on TNF-α induced NF-κB dependent reporter gene expression. Transfected cells were treated with vehicle or PMS601 (20, 40, 60 and 80 µM), then incubated with TNF-α (20 ng/ml) for 12 h. After treatment, luciferase activity in (A), (B), (C), and (D) were measured using a dual luciferase assay system (Promega). Data shown represent the relative luciferase activity normalized against Renilla luciferase activity. The values are the mean±S.D. for three independent replicates. *<i>P</i><0.05 versus TNF-α stimulated group; NS, not significant.