<p>CB6F1 mice were vaccinated once with rH4 in cationic liposomes followed two weeks later by one Ad-H4 vaccination, and challenged by the aerosol route with virulent <i>M.tb</i> at week 10. The last three days before killing, BrdU (0.8 mg/ml) was added to the drinking water. Two weeks after challenge, mice were sacrificed and lung lymphocytes were stimulated with Ag85B or TB10.4 peptides prior to staining with anti-CD4, -CD8, -BrdU, and -IFNγ (A–C). (A) Frequencies represent the percentage of BrdU<sup>+</sup> CD4 or CD8 T cells out of total CD4 or CD8 T cells. Data represent the mean of +SEM of four mice per group with *, p<0.05 (Unpaired t-test, two-tailed). Dot plots, representative of four mice, are also shown depicting BrdU<sup>+</sup>...
<p>(A) the amino acid sequence of the TB10.4 protein with the CD4 and CD8 T cell epitopes underlined...
<p><b>(a)</b> The TB10 tetramer<sup>+</sup> response enumerated by duel-tetramer staining in blood 1...
<p>The specific lysis of TB10.4<sub>3-11</sub>, TB10.4<sub>20-28</sub>, and TB10.4<sub>70-88</sub> l...
<p>Antigen specific CD4 and CD8 T cells in rH4/Ad-H4 vaccinated mice were measured two weeks before ...
<p>CB6F1 mice were vaccinated once with Ad-H4, twice with rH4 in cationic liposomes, or once with rH...
<p>CB6F1 mice were vaccinated once with Ad-H4, twice with rH4 in cationic liposomes, or once with rH...
<p>(Early: week 6; Intermediate: week 16, late: week 40) (A and B) Lung cells from infected mice wer...
<p>Frequency of cytokine producing Ag85B and TB10.4 specific CD4 and CD8 T cells two weeks before an...
<p>(A and B) CB6F1 mice were infected by the aerosol route with virulent <i>M.tb Erdman</i> and lymp...
<p>The protocol of the experiment was as described in the legend of <a href="http://www.plospathogen...
<p>C57BL/6 and CBA/J mice were infected with an aerosolized dose of <i>Mtb</i> and at various timepo...
<p>(A) Mice (n = 4) from different mouse strains were infected with 10<sup>5</sup> pfu MCMV. Blood s...
<p>Each group of mice was immunized and sacrificed as described in the materials and methods section...
<p>A. Total lung cell numbers of vaccinia vaccinated and mock (PBS) vaccinated mice following bleomy...
<p>(<b>a</b>) Clonality of CD8+ TCR sequences obtained from infected lung granulomas (n = 6) compare...
<p>(A) the amino acid sequence of the TB10.4 protein with the CD4 and CD8 T cell epitopes underlined...
<p><b>(a)</b> The TB10 tetramer<sup>+</sup> response enumerated by duel-tetramer staining in blood 1...
<p>The specific lysis of TB10.4<sub>3-11</sub>, TB10.4<sub>20-28</sub>, and TB10.4<sub>70-88</sub> l...
<p>Antigen specific CD4 and CD8 T cells in rH4/Ad-H4 vaccinated mice were measured two weeks before ...
<p>CB6F1 mice were vaccinated once with Ad-H4, twice with rH4 in cationic liposomes, or once with rH...
<p>CB6F1 mice were vaccinated once with Ad-H4, twice with rH4 in cationic liposomes, or once with rH...
<p>(Early: week 6; Intermediate: week 16, late: week 40) (A and B) Lung cells from infected mice wer...
<p>Frequency of cytokine producing Ag85B and TB10.4 specific CD4 and CD8 T cells two weeks before an...
<p>(A and B) CB6F1 mice were infected by the aerosol route with virulent <i>M.tb Erdman</i> and lymp...
<p>The protocol of the experiment was as described in the legend of <a href="http://www.plospathogen...
<p>C57BL/6 and CBA/J mice were infected with an aerosolized dose of <i>Mtb</i> and at various timepo...
<p>(A) Mice (n = 4) from different mouse strains were infected with 10<sup>5</sup> pfu MCMV. Blood s...
<p>Each group of mice was immunized and sacrificed as described in the materials and methods section...
<p>A. Total lung cell numbers of vaccinia vaccinated and mock (PBS) vaccinated mice following bleomy...
<p>(<b>a</b>) Clonality of CD8+ TCR sequences obtained from infected lung granulomas (n = 6) compare...
<p>(A) the amino acid sequence of the TB10.4 protein with the CD4 and CD8 T cell epitopes underlined...
<p><b>(a)</b> The TB10 tetramer<sup>+</sup> response enumerated by duel-tetramer staining in blood 1...
<p>The specific lysis of TB10.4<sub>3-11</sub>, TB10.4<sub>20-28</sub>, and TB10.4<sub>70-88</sub> l...