The kinetics of CD4/10.4 and CD8/10.4 following aerosol infection with <i>M.tb</i>.

<p>(A and B) CB6F1 mice were infected by the aerosol route with virulent <i>M.tb Erdman</i> and lymphocytes were obtained from lungs or blood and then stimulated with TB10.4 _3–11 or TB10.4 _74–88 for assessment of IFN-γ production by FACS analysis. Frequencies represent IFN-γ production out of total T cells. Background staining from cells stimulated with medium alone has been deducted (Background <0.5%). Each time point represents the mean from at least three individual mice±standard error of the mean (SEM). (C) Lung or blood cells were stained directly <i>ex vivo</i> with H2-<i>K^b</i> pentamer loaded with IMYNYPAM. Background staining from naïve mice has been deducted. Each time point consists of data from a pool of 3–6 mice. (**p<0.01, Student's t-test).