Frequency of T cell subsets.

<p>Frequency of cytokine producing Ag85B and TB10.4 specific CD4 and CD8 T cells two weeks before and after <i>M.tb</i> challenge, as well as the frequencies of total CD4 and CD8 T cells, in rH4/Ad-H4 vaccinated mice.</p>*<p>The frequencies were obtained by FACS analysis on <i>in vitro</i> antigen stimulated splenocyte cultures from vaccinated mice (two weeks before challenge).</p>**<p>The frequencies were obtained by FACS analysis on <i>in vitro</i> antigen stimulated lung lymphocyte cultures from vaccinated and <i>M.tb</i> challenged mice two weeks after challenge.</p>***<p>Background staining from cells stimulated with medium alone has been subtracted.