Recruitment of Ag85B and TB10.4 CD4 and CD8 T cells to the lung.

<p>Antigen specific CD4 and CD8 T cells in rH4/Ad-H4 vaccinated mice were measured two weeks before and after challenge. CB6F1 mice were vaccinated once with rH4 in cationic liposomes followed two weeks later by one Ad-H4 vaccination, and challenged by the aerosol route with virulent <i>M.tb</i> six weeks after the final vaccination. Two weeks before challenge and two weeks after challenge mice were sacrificed, and splenocytes and lung lymphocytes, respectively, were stimulated with Ag85B or TB10.4 peptides prior to staining with anti-CD4, -CD8, -CD44, -IFN-γ, -IL-2, and –TNF-α. (A–D) The number of cytokine producing T cells is a total number of triple, double, and single positive IFN-γ, TNF-α, and IL-2 producing antigen specific CD4 or CD8 T cells either per spleen pre-infection (A and B) or per lung post infection (C and D). Background staining from cells stimulated with medium alone has been subtracted. Data represent the mean+SEM of four mice per group with **, p<0.01 (2-way ANOVA with Bonferroni posttest). (E and F) The ratio between Ag85B (E) and TB10.4 (F) specific CD4 and CD8 T cells was calculated from A–D.