<p>A: Schematic representation of the culture procedure used to derive the RYAS41 cell line. P represents passage number. Surface of the culture well is indicated below the time line. B: Coexpression of insulin (red) and SV40T (green); insulin (red) and c-peptide (green), insulin (red) and Pdx1 (green); and double staining for insulin (red) and BrdU (green). Scale bars: 25 µm.</p
<p>(A-B) Immunofluorescence images of INS-1E (A) and MIN6 (B) beta cell lines. Left images represent...
<p>a–c: insulin secretion after 1, 10, and 20 day cultures, respectively. (mean±SEM: n = 3, *:p<0.05...
<p>Parameters were obtained from the biochemical models at steady state for each of the four cell li...
<p>A–C: Staining for insulin (red), SV40T (green) and DAPI (blue); D–F: Staining for insulin (red), ...
<p>(a, b, e, f, i, j, m, n, q, r, u, v) Representative images taken of immunostained islets at 6, 12...
<p>(A) At Day 14, the differentiated hESCs were positively stained with antibodies against glucagon....
<p>A: Semi-quantitative RT PCR comparison between RYAS41, lung (negative control) and pancreas (posi...
SR1423 differentiation yields robust pancreatic, hormone-secreting cell populations. (A) SR1423 diff...
hiPS-RPE cell sheets were cultured for 49 days using both machine and manual culture methods. (A) Sc...
<p>(A) Triple staining of insulin (green), PICK1 (red), and ICA69 (blue) on INS-1E cells. Arrowheads...
<p>(A) Schematic representation of aggregation and the four-stage differentiation protocol from hESC...
<p>A: Conventional X-gal staining of an islet. Note both the cytosolic and granular staining. B: Ele...
<p>(A) Representative flow cytometric profiles of cells at passage 3, showing the percentage of cell...
<p>A–C: Section of adult mouse pancreas stained for glucagon (green) and insulin (red). D–F: Section...
<p>(A) Microscopic images of C57BL/6-derived pSGCs (upper, first panel), control mMSCs (lower, first...
<p>(A-B) Immunofluorescence images of INS-1E (A) and MIN6 (B) beta cell lines. Left images represent...
<p>a–c: insulin secretion after 1, 10, and 20 day cultures, respectively. (mean±SEM: n = 3, *:p<0.05...
<p>Parameters were obtained from the biochemical models at steady state for each of the four cell li...
<p>A–C: Staining for insulin (red), SV40T (green) and DAPI (blue); D–F: Staining for insulin (red), ...
<p>(a, b, e, f, i, j, m, n, q, r, u, v) Representative images taken of immunostained islets at 6, 12...
<p>(A) At Day 14, the differentiated hESCs were positively stained with antibodies against glucagon....
<p>A: Semi-quantitative RT PCR comparison between RYAS41, lung (negative control) and pancreas (posi...
SR1423 differentiation yields robust pancreatic, hormone-secreting cell populations. (A) SR1423 diff...
hiPS-RPE cell sheets were cultured for 49 days using both machine and manual culture methods. (A) Sc...
<p>(A) Triple staining of insulin (green), PICK1 (red), and ICA69 (blue) on INS-1E cells. Arrowheads...
<p>(A) Schematic representation of aggregation and the four-stage differentiation protocol from hESC...
<p>A: Conventional X-gal staining of an islet. Note both the cytosolic and granular staining. B: Ele...
<p>(A) Representative flow cytometric profiles of cells at passage 3, showing the percentage of cell...
<p>A–C: Section of adult mouse pancreas stained for glucagon (green) and insulin (red). D–F: Section...
<p>(A) Microscopic images of C57BL/6-derived pSGCs (upper, first panel), control mMSCs (lower, first...
<p>(A-B) Immunofluorescence images of INS-1E (A) and MIN6 (B) beta cell lines. Left images represent...
<p>a–c: insulin secretion after 1, 10, and 20 day cultures, respectively. (mean±SEM: n = 3, *:p<0.05...
<p>Parameters were obtained from the biochemical models at steady state for each of the four cell li...